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Biophysics of RNA-Protein Interactions

Biophysics of RNA-Protein Interactions PDF Author: Chirlmin Joo
Publisher: Springer Nature
ISBN: 1493997262
Category : Science
Languages : en
Pages : 249
Book Description
RNA molecules play key roles in all aspects of cellular life, but to do so efficiently, they must work in synergism with proteins. This book addresses how proteins and RNA interact to carry out biological functions such as protein synthesis, regulation of gene expression, genome defense, liquid phase separation and more. The topics addressed in this volume will appeal to researchers in biophysics, biochemistry and structural biology. The book is a useful resource for anybody interested in elucidating the molecular mechanisms and discrete properties of RNA-protein complexes. Included are reviews of key systems such as microRNA and CRISPR/Cas that exemplify how RNA and proteins work together to perform their biological function. Also covered are techniques ranging from single molecule fluorescence and force spectroscopy to crystallography, cryo-EM microscopy, and kinetic modeling.

Biophysics of RNA-Protein Interactions

Biophysics of RNA-Protein Interactions PDF Author: Chirlmin Joo
Publisher: Springer Nature
ISBN: 1493997262
Category : Science
Languages : en
Pages : 249
Book Description
RNA molecules play key roles in all aspects of cellular life, but to do so efficiently, they must work in synergism with proteins. This book addresses how proteins and RNA interact to carry out biological functions such as protein synthesis, regulation of gene expression, genome defense, liquid phase separation and more. The topics addressed in this volume will appeal to researchers in biophysics, biochemistry and structural biology. The book is a useful resource for anybody interested in elucidating the molecular mechanisms and discrete properties of RNA-protein complexes. Included are reviews of key systems such as microRNA and CRISPR/Cas that exemplify how RNA and proteins work together to perform their biological function. Also covered are techniques ranging from single molecule fluorescence and force spectroscopy to crystallography, cryo-EM microscopy, and kinetic modeling.

Biophysics of DNA-Protein Interactions

Biophysics of DNA-Protein Interactions PDF Author: Mark C. Williams
Publisher: Springer Science & Business Media
ISBN: 0387928081
Category : Science
Languages : en
Pages : 350
Book Description
Depite the rapid expansion of the field of biophysics, there are very few books that comprehensively treat specific topics in this area. Recently, the field of single molecule biophysics has developed very quickly, and a few books specifically treating single molecule methods are beginning to appear. However, the promise of single molecule biophysics is to contribute to the understanding of specific fields of biology using new methods. This book would focus on the specific topic of the biophysics of DNA-protein interactions, and would include the use of new approaches, including both bulk methods as well as single molecule methods. This would make the book attractive to anyone working in the general area of DNA-protein interactions, which is of course a much wider market than just single molecule biophysicists or even biophysicists. The subject of the book will be the biophysics of DNA-protein interactions, and will include new methods and results that describe the physical mechanism by which proteins interact with DNA. For example, there has been much recent work on the mechanism by which proteins search for specific binding sites on DNA. A few chapters will be devoted to experiments and theory that shed light on this important problem. We will also cover proteins that alter DNA properties to facilitate interactions important for transcription or replication. Another section of the book will cover the biophysical mechanism by which motor proteins interact with DNA. Finally, we will cover larger protein-DNA complexes, such as replication forks, recombination complexes, DNA repair interactions, and their chromatin context.

An Analysis of the Molecular Basis of TRNA-protein Interactions

An Analysis of the Molecular Basis of TRNA-protein Interactions PDF Author: Joseph John Rosa
Publisher:
ISBN:
Category : Proteins
Languages : en
Pages : 400
Book Description


Introduction to Biomolecular Structure and Biophysics

Introduction to Biomolecular Structure and Biophysics PDF Author: Gauri Misra
Publisher: Springer
ISBN: 9811049688
Category : Medical
Languages : en
Pages : 274
Book Description
This comprehensive book presents a modern concept in biophysics based on recently published research. It highlights various aspects of the biophysical fundamentals and techniques that are currently used to study different physical properties of biomolecules, and relates the biological phenomenon with the underlying physical concepts. The content is divided into nine chapters summarizing the structural details of proteins, including recently discovered novel folds, higher order structures of nucleic acids, as well as lipids and the physical forces governing the macromolecular interactions which are essential for the various biological processes. It also provides insights into the recent advances in biophysical techniques including Hydrogen Deuterium Exchange with Mass Spectrometry (HDX-MS), Small angle X-ray scattering (SAXS) and Cryo Electron Microscopy (cryo EM), supplemented with interesting experimental data. It is a valuable reference resource for anyone with a desire to gain a better understanding of the fundamentals of biophysical concepts and techniques of important biomolecules.

RNA-Protein Interaction Protocols[

RNA-Protein Interaction Protocols[ PDF Author: Susan R. Haynes
Publisher: Springer Science & Business Media
ISBN: 1592596762
Category : Science
Languages : en
Pages : 481
Book Description
In RNA-Protein Interaction Protocols, highly skilled experimentalists provide a comprehensive series of commonly used, as well as specialized, techniques for analyzing how proteins and RNA interact. Richly detailed and readily reproducible, these methods enable researchers to analyze the structural details of an RNA-protein interaction, to determine in detail what parts of the protein and RNA are in close contact, and to isolate RNP complexes from cells. There are also in vitro and in vivo methods and protocols for assaying the effects of proteins and RNP complexes on mRNA metabolism. The methods assume only a knowledge of basic molecular biology, biochemistry, and cell culture, and can be readily adapted to other systems. Providing an excellent standard resource for today's bench scientists studying many types of RNA-protein interactions, RNA-Protein Interaction Protocols offers a wide-ranging collection of up-to-date experimental methods that distill a great deal of wisdom and experience into productive, reproducible, step-by-step techniques for all investigators in the field.

Biophysical approaches to translational control of gene expression

Biophysical approaches to translational control of gene expression PDF Author: Jonathan D. Dinman
Publisher: Springer Science & Business Media
ISBN: 1461439914
Category : Medical
Languages : en
Pages : 320
Book Description
This book provides a premier resource on understanding the ribosome's essential nature and how it interacts with other proteins and nucleic acids to control protein synthesis. As one of the central foundations in our understanding of the biology at the molecular level, this topic appeals to a wide audience, from bench researcher to clinician. With the advent of atomic scale structures, methods to visualize and separate individual molecules, and the computational power to model the complex interactions of over a million atoms at once, our understanding of how gene expression is controlled at the level of protein translation is now deeply ensconced in the biophysical realm.

Protein-binding and RNA-binding Properties of RNA-binding Proteins FBF and RBM39

Protein-binding and RNA-binding Properties of RNA-binding Proteins FBF and RBM39 PDF Author: Joann Wu
Publisher:
ISBN: 9781303073212
Category : Biochemistry
Languages : en
Pages : 432
Book Description
Post-transcriptional regulation of gene expression is critical in the development, physiology, and reproduction of multicellular organisms. Large dynamic multi-protein complexes formed through extensive protein*protein and protein*RNA interactions control mRNA processing, splicing, nuclear transport, localization, stability, and translation. However, despite the biological importance of these regulatory complexes, there is sparse molecular information describing the interactions involved in their assembly. In these studies, two categories of RNA-binding proteins that act as foundations for these complexes are examined: the Pu milio and F[barbelow]BF (PUF) proteins and R[barbelow]NA r[barbelow]ecognition m[barbelow]otif (RRM) domain-containing proteins. While there is a wealth of knowledge for PUF protein interactions with RNA, there is limited information describing the equally essential interactions between PUF proteins and their protein binding partners. Defined in these studies are the molecular bases for the developmentally essential interactions between Caenorhabditis elegans PUF protein f[barbelow]em-3 b[barbelow]inding f[barbelow]actor (FBF) and the two protein binding partners: g[barbelow]erml[barbelow]ine d[barbelow]evelopment defective-3[barbelow] (GLD-3) and c[barbelow]ytoplasmic p[barbelow]olyadenylation element b[barbelow]inding-1 (CPB-1). Short specific sequences in unstructured regions of the GLD-3 and CPB-1 proteins are critical for interaction with FBF, while a loop connecting PUF repeats R7 and R8 in FBF is essential for association with both GLD-3 and CPB-1. The PUF protein*binding partner complex FBF*GLD-3 is capable of forming a stable complex on RNA FBF regulatory elements in the 3' u[barbelow]nt[barbelow]ranslated r[barbelow]egions (UTR) of specific germline developmental mRNAs, and may control their expression through several proposed mechanisms. Although the RRM domains that characterize RRM-containing proteins adopt the same vary greatly in: amino acid sequence; DNA, RNA, and protein-binding abilities; and mode of interaction with these biological molecules. These studies potentially identify the first member of a unique class of RRMs that bind to RNA in a manner similar to RRMs that interact with proteins. These studies characterizing the protein- and RNA-binding properties of these RNA-binding proteins provide insight into the biologically essential molecular complexes involved in post-transcriptional gene regulation.

Structures of Large RNA Molecules and Their Complexes

Structures of Large RNA Molecules and Their Complexes PDF Author:
Publisher: Academic Press
ISBN: 0128019360
Category : Science
Languages : en
Pages : 654
Book Description
This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in RNA folding and dynamics, RNA-protein interactions and large RNPs. Continues the legacy of this premier serial with quality chapters on structures of large RNA molecules and their complexes

Issues in Biochemistry and Biophysics Research: 2013 Edition

Issues in Biochemistry and Biophysics Research: 2013 Edition PDF Author:
Publisher: ScholarlyEditions
ISBN: 1490108653
Category : Science
Languages : en
Pages : 1218
Book Description
Issues in Biochemistry and Biophysics Research: 2013 Edition is a ScholarlyEditions™ book that delivers timely, authoritative, and comprehensive information about Amino Acids. The editors have built Issues in Biochemistry and Biophysics Research: 2013 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Amino Acids in this book to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Issues in Biochemistry and Biophysics Research: 2013 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.

Biophysics

Biophysics PDF Author: Mark C. Leake
Publisher: CRC Press
ISBN: 1498702465
Category : Medical
Languages : en
Pages : 390
Book Description
An Up-to-Date Toolbox for Probing Biology Biophysics: Tools and Techniques covers the experimental and theoretical tools and techniques of biophysics. It addresses the purpose, science, and application of all physical science instrumentation and analysis methods used in current research labs. The book first presents the historical background, concepts, and motivation for using a physical science toolbox to understand biology. It then familiarizes students from the physical sciences with essential biological knowledge. The text subsequently focuses on experimental biophysical techniques that primarily detect biological components or measure/control biological forces. The author describes the science and application of key tools used in imaging, detection, general quantitation, and biomolecular interaction studies, which span multiple length and time scales of biological processes both in the test tube and in the living organism. Moving on to theoretical biophysics tools, the book presents computational and analytical mathematical methods for tackling challenging biological questions. It concludes with a discussion of the future of this exciting field. Future innovators will need to be trained in multidisciplinary science to be successful in industry, academia, and government support agencies. Addressing this challenge, this textbook educates future leaders on the development and application of novel physical science approaches to solve complex problems linked to biological questions.