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Invertebrate Tissue Culture Methods

Invertebrate Tissue Culture Methods PDF Author: Jun Mitsuhashi
Publisher: Springer Science & Business Media
ISBN: 9784431703136
Category : Science
Languages : en
Pages : 472
Book Description
The techniques for establishing and maintaining invertebrate tissues and cells in culture remain difficult due to the diversity of invertebrates and their structural and physiological characteristics. Research involving invertebrate cell cultures continues to increase, although the number of cell lines used is still limited. This manual gives detailed descriptions of the technical procedures for the establishment of primary invertebrate cell cultures in vitro. Nutritional requirements, culture media, and species-specific methods for both cell and organ cultures as well as useful techniques for studies on cultured cells are described. The Appendix lists established cell lines available for research with information on the composition of their physiological and nutrient solutions. This comprehensive manual, the first of its kind, is a valuable reference for investigators working with invertebrate cell cultures in academia and industry.

Invertebrate Tissue Culture Methods

Invertebrate Tissue Culture Methods PDF Author: Jun Mitsuhashi
Publisher: Springer Science & Business Media
ISBN: 9784431703136
Category : Science
Languages : en
Pages : 472
Book Description
The techniques for establishing and maintaining invertebrate tissues and cells in culture remain difficult due to the diversity of invertebrates and their structural and physiological characteristics. Research involving invertebrate cell cultures continues to increase, although the number of cell lines used is still limited. This manual gives detailed descriptions of the technical procedures for the establishment of primary invertebrate cell cultures in vitro. Nutritional requirements, culture media, and species-specific methods for both cell and organ cultures as well as useful techniques for studies on cultured cells are described. The Appendix lists established cell lines available for research with information on the composition of their physiological and nutrient solutions. This comprehensive manual, the first of its kind, is a valuable reference for investigators working with invertebrate cell cultures in academia and industry.

Invertebrate Tissue Culture

Invertebrate Tissue Culture PDF Author: C Vago
Publisher: Elsevier
ISBN: 0323149669
Category : Science
Languages : en
Pages : 456
Book Description
Invertebrate Tissue Culture, Volume I, reviews advances in the use of tissue and organ culture in invertebrate research in physiology and pathology. It describes methods in invertebrate tissue culture, including organ culture techniques in liquid and gel media; aseptic rearing of invertebrates for tissue culture; and cell culture of organisms, ranging from Lepidoptera and Diptera to Coleoptera, Orthoptera, Dictyoptera, Hymenoptera, Hemiptera, Crustacea, Arachnida, Merostomacea, and mollusks. Organized into two sections encompassing 13 chapters, this volume begins with an overview of the general methodology in cell and organ cultures and their preparation from aseptic conditions. It then discusses methods for the examination of cultures, including those concerning ultrastructure studies by electron microscopy. The reader is also introduced to cell cultures obtained from different groups of invertebrates, with emphasis on peculiarities specific to each group; the morphology and physiology of cultured invertebrate cells; and cultivation and growth of cells. This book is a valuable resource for specialists in the field of invertebrate cell and organ culture.

Tissue Culture: Methods and Applications

Tissue Culture: Methods and Applications PDF Author: Paul F. Kruse
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 904
Book Description
Tissue Culture presents a series of protocols useful for dissociating tissues followed by exemplary procedures for establishing primary cultures of cells from vertebrate, invertebrate, and plant sources. Methodology in tissue culture has progressed dramatically, and much of this gain has not been summarized in book form. This book attempts to present an overview of up-to-date procedures for working with cells in culture and for using them in a wide variety of scientific disciplines. The book is divided into 14 sections, with the first focusing on primary tissue dissociation. The succeeding sec ...

Invertebrate Tissue Culture

Invertebrate Tissue Culture PDF Author: Edouard Kurstak
Publisher: Elsevier
ISBN: 0323151469
Category : Science
Languages : en
Pages : 413
Book Description
Invertebrate Tissue Culture: Applications in Medicine, Biology, and Agriculture comprises the proceedings of the IV International Conference on Invertebrate Tissue Culture, held on June 5-8, 1975 at Mont Gabriel, Quebec, Canada. The conference focuses on invertebrate organ, tissue, and cell culture, as well as cell culture limitations, pitfalls, and applications in medicine, biology, agriculture, neurophysiology, and studies of morphogenesis, differentiation, viruses, symbionts, and parasites. This reference material specifically provides information on sophisticated laboratory methods and on numerous utilizations of invertebrate cell culture techniques in medicine and biology. This book also elucidates the nutritional requirements and the establishment of cell lines. The study of viruses and protozoa of agricultural and forest importance is also shown. This book will be useful and stimulating to the readers and will provide in a single volume the results obtained in the diverse areas of research pursued by the leading exponents of invertebrate tissue culture from America, Europe, Asia, and Australia.

Invertebrate Tissue Culture

Invertebrate Tissue Culture PDF Author: Noriaki Agui
Publisher:
ISBN:
Category : Cultures (Biology)
Languages : en
Pages : 416
Book Description


Invertebrate Cell System Applications

Invertebrate Cell System Applications PDF Author: Jun Mitsuhashi
Publisher: CRC Press
ISBN: 1351090747
Category : Science
Languages : en
Pages : 253
Book Description
A useful reference for those using or interested in cultured invertebrate cells, this two-volume text provides information about techniques and advances in invertebrate tissue culture. Cell lines for Insecta, Crustacea, Mollusca, and Nematoda are introduces along with their characterizations. Developments in insect biotechnology, including foreign protein production by insect cells infected with recombinant virus are described. Fundamental studies for introducing foreign genes into cultured insect cells is also presented. Wide information on studies -at cellular levels-on pathogens of insects, plants, and vertebrates is given.

Aquatic Invertebrate Cell Culture

Aquatic Invertebrate Cell Culture PDF Author: Carmel Mothersil
Publisher: Springer
ISBN:
Category : Medical
Languages : en
Pages : 446
Book Description
Aquatic Invertebrate Cell Culture is a very new field which has major applications in Aquaculture, Ecotoxicology and Pathology. In essence it is realised that pathogens behave differently in host cells than the impression gained from growth on agar plates. Another major application of invertebrate cell culture is an understanding of mechanisms involved in cellular and molecular responses to environmental change. This book aims to consider all relevant advances for the development of aquatic invertebrate cell culture.

Invertebrate Tissue Culture Methods

Invertebrate Tissue Culture Methods PDF Author: Jun Mitsuhashi
Publisher: Springer Science & Business Media
ISBN: 4431678751
Category : Science
Languages : en
Pages : 446
Book Description
I started insect cell culture work in 1962, when T. D. C. Grace reported the first establishment of invertebrate continuous cell lines. He obtained grow ing cells from pupal ovaries of the emperor gum moth, Antheraea euca lypti. At that time, I was trying to obtain growing cells from leafhoppers. Grace's method could not be applied directly to my culture because of the differences in species, the size of the insects, and the tissue to be cul tured. The vertebrate tissue culture methods gave me some ideas for pre paring cultures from leafhoppers, but those could not be used directly either. There were no textbooks and no manuals for invertebrate tissue culture, so I had to develop a method by myself. First, I considered what type and what size of vessels are suitable for insect tissue culture. Also, I had to look for suitable materials to construct the culture vessels. Sec ond, I had to examine various culture media, especially growth-promot ing substances, such as sera. Then I had to improve culture media by trial and error. The procedure to set up a primary culture was also a problem. How could I sterilize materials? How could I remove tissues from a tiny insect? How many tissues should I pool in order to set up one culture? I had to find out the answers. Naturally, it took a lot of time.

Invertebrate and Fish Tissue Culture

Invertebrate and Fish Tissue Culture PDF Author: Yukiaki Kuroda
Publisher: Springer Verlag
ISBN:
Category : Cell culture
Languages : en
Pages : 316
Book Description
The sessions dealt with new cultures and techniques, physiology and endocrinology of cells in vitro, production of specific proteins in vitro, cell differentiation and gene expression, application of cell cultures to pathology and microbiology, fish cell culture development and application, and new cell lines and their characterization.

Invertebrate Cell System Applications, Volume II

Invertebrate Cell System Applications, Volume II PDF Author: Jun Mitsuhashi
Publisher: CRC Press
ISBN:
Category : Science
Languages : en
Pages : 330
Book Description
A useful reference for those using or interested in cultured invertebrate cells, this two-volume text provides information about techniques and advances in invertebrate tissue culture. Cell lines for Insecta, Crustacea, Mollusca, and Nematoda are introduces along with their characterizations. Developments in insect biotechnology, including foreign protein production by insect cells infected with recombinant virus are described. Fundamental studies for introducing foreign genes into cultured insect cells is also presented. Wide information on studies -at cellular levels-on pathogens of insects, plants, and vertebrates is given.