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Invertebrate Tissue Culture Methods

Invertebrate Tissue Culture Methods PDF Author: Jun Mitsuhashi
Publisher: Springer Science & Business Media
ISBN: 4431678751
Category : Science
Languages : en
Pages : 446
Book Description
I started insect cell culture work in 1962, when T. D. C. Grace reported the first establishment of invertebrate continuous cell lines. He obtained grow ing cells from pupal ovaries of the emperor gum moth, Antheraea euca lypti. At that time, I was trying to obtain growing cells from leafhoppers. Grace's method could not be applied directly to my culture because of the differences in species, the size of the insects, and the tissue to be cul tured. The vertebrate tissue culture methods gave me some ideas for pre paring cultures from leafhoppers, but those could not be used directly either. There were no textbooks and no manuals for invertebrate tissue culture, so I had to develop a method by myself. First, I considered what type and what size of vessels are suitable for insect tissue culture. Also, I had to look for suitable materials to construct the culture vessels. Sec ond, I had to examine various culture media, especially growth-promot ing substances, such as sera. Then I had to improve culture media by trial and error. The procedure to set up a primary culture was also a problem. How could I sterilize materials? How could I remove tissues from a tiny insect? How many tissues should I pool in order to set up one culture? I had to find out the answers. Naturally, it took a lot of time.

Invertebrate Tissue Culture Methods

Invertebrate Tissue Culture Methods PDF Author: Jun Mitsuhashi
Publisher: Springer Science & Business Media
ISBN: 4431678751
Category : Science
Languages : en
Pages : 446
Book Description
I started insect cell culture work in 1962, when T. D. C. Grace reported the first establishment of invertebrate continuous cell lines. He obtained grow ing cells from pupal ovaries of the emperor gum moth, Antheraea euca lypti. At that time, I was trying to obtain growing cells from leafhoppers. Grace's method could not be applied directly to my culture because of the differences in species, the size of the insects, and the tissue to be cul tured. The vertebrate tissue culture methods gave me some ideas for pre paring cultures from leafhoppers, but those could not be used directly either. There were no textbooks and no manuals for invertebrate tissue culture, so I had to develop a method by myself. First, I considered what type and what size of vessels are suitable for insect tissue culture. Also, I had to look for suitable materials to construct the culture vessels. Sec ond, I had to examine various culture media, especially growth-promot ing substances, such as sera. Then I had to improve culture media by trial and error. The procedure to set up a primary culture was also a problem. How could I sterilize materials? How could I remove tissues from a tiny insect? How many tissues should I pool in order to set up one culture? I had to find out the answers. Naturally, it took a lot of time.

Tissue Culture: Methods and Applications

Tissue Culture: Methods and Applications PDF Author: Paul F. Kruse
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 904
Book Description
Tissue Culture presents a series of protocols useful for dissociating tissues followed by exemplary procedures for establishing primary cultures of cells from vertebrate, invertebrate, and plant sources. Methodology in tissue culture has progressed dramatically, and much of this gain has not been summarized in book form. This book attempts to present an overview of up-to-date procedures for working with cells in culture and for using them in a wide variety of scientific disciplines. The book is divided into 14 sections, with the first focusing on primary tissue dissociation. The succeeding sec ...

Invertebrate Tissue Culture

Invertebrate Tissue Culture PDF Author: C Vago
Publisher: Academic Press
ISBN: 1483266923
Category : Science
Languages : en
Pages : 430
Book Description
Invertebrate Tissue Culture, Volume II presents the organ cultures of various invertebrates. This book discusses the use of cell and organ cultures in genetics, physiology, and pathology. Organized into three parts encompassing 11 chapters, this volume begins with an overview of the factors necessary for the normal development of embryonic or imaginal rudiments. This text then examines the organ culture technique, which is a means of studying the correlations that may exist between various organs as they can be made to act on one another in the same medium independently of any other factor. Other chapters consider a detailed analysis of the utilization of organ culture in pathology. This book discusses as well the three major successive aspects of mollusk organ cultures. The final chapter provides information on cell lines from 35 species of insects from various orders and from one species of mollusk. This book is a valuable resource for biologists, pathologists, and physiologists.

Invertebrate Tissue Culture

Invertebrate Tissue Culture PDF Author: Edouard Kurstak
Publisher: Elsevier
ISBN: 0323151469
Category : Science
Languages : en
Pages : 413
Book Description
Invertebrate Tissue Culture: Applications in Medicine, Biology, and Agriculture comprises the proceedings of the IV International Conference on Invertebrate Tissue Culture, held on June 5-8, 1975 at Mont Gabriel, Quebec, Canada. The conference focuses on invertebrate organ, tissue, and cell culture, as well as cell culture limitations, pitfalls, and applications in medicine, biology, agriculture, neurophysiology, and studies of morphogenesis, differentiation, viruses, symbionts, and parasites. This reference material specifically provides information on sophisticated laboratory methods and on numerous utilizations of invertebrate cell culture techniques in medicine and biology. This book also elucidates the nutritional requirements and the establishment of cell lines. The study of viruses and protozoa of agricultural and forest importance is also shown. This book will be useful and stimulating to the readers and will provide in a single volume the results obtained in the diverse areas of research pursued by the leading exponents of invertebrate tissue culture from America, Europe, Asia, and Australia.

Invertebrate Cell Cultures

Invertebrate Cell Cultures PDF Author: W. F. Hink
Publisher:
ISBN:
Category : Cell culture
Languages : en
Pages : 46
Book Description


Invertebrate Cell System Applications

Invertebrate Cell System Applications PDF Author: Jun Mitsuhashi
Publisher: CRC Press
ISBN: 9781315894751
Category :
Languages : en
Pages : 322
Book Description
A useful reference for those using or interested in cultured invertebrate cells, this two-volume text provides information about techniques and advances in invertebrate tissue culture. Cell lines for Insecta, Crustacea, Mollusca, and Nematoda are introduces along with their characterizations. Developments in insect biotechnology, including foreign protein production by insect cells infected with recombinant virus are described. Fundamental studies for introducing foreign genes into cultured insect cells is also presented. Wide information on studies -at cellular levels-on pathogens of insects, plants, and vertebrates is given.

Invertebrate Tissue Culture

Invertebrate Tissue Culture PDF Author: C. Vago
Publisher:
ISBN:
Category : Cultures (Biology)
Languages : en
Pages : 476
Book Description


Invertebrate Tissue Culture

Invertebrate Tissue Culture PDF Author: Université de Montréal
Publisher: New York : Academic Press
ISBN:
Category : Congresses
Languages : en
Pages : 424
Book Description


Invertebrate Cell System Applications

Invertebrate Cell System Applications PDF Author: Jun Mitsuhashi
Publisher: CRC Press
ISBN: 1351090747
Category : Science
Languages : en
Pages : 253
Book Description
A useful reference for those using or interested in cultured invertebrate cells, this two-volume text provides information about techniques and advances in invertebrate tissue culture. Cell lines for Insecta, Crustacea, Mollusca, and Nematoda are introduces along with their characterizations. Developments in insect biotechnology, including foreign protein production by insect cells infected with recombinant virus are described. Fundamental studies for introducing foreign genes into cultured insect cells is also presented. Wide information on studies -at cellular levels-on pathogens of insects, plants, and vertebrates is given.

Invertebrate Tissue Culture

Invertebrate Tissue Culture PDF Author: Noriaki Agui
Publisher:
ISBN:
Category : Cultures (Biology)
Languages : en
Pages : 416
Book Description